also published in The New England Journal of Medicine. Concerns are raised regarding integration of lentivirus into the host genome. Previous studies have linked lentiviral vectors to blood cancer ...

A new vector system known as lentiviral vectors can permanently integrate the gene into dividing and non-dividing cells. The capacity of lentiviral vectors to reliably integrate at various genomic ...

Here we report a case of a T-cell lymphoma harboring a lentiviral integration in a known tumor ... CAR was detected in high abundance in DNA extracted from the patient’s duodenal-biopsy samples ...

The findings overturn the long-held idea that most types of RNA viruses are incapable of DNA integration and raise another safety concern in the use of RNA-based gene therapy. "It's a very interesting ...

Monoclonal T cells from both patients had detectable CAR transgene expression and integration ... T-cell therapy that is produced by means of lentiviral transduction. In the phase 3, randomized ...

4 Finally, Largaespada noted that Davis and his team have made both vectors and cell lines with already-integrated SSR sites available to other scientists: “I think it will be quite easy to [bring in] ...

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and ... editing in different mammalian cell lines for research purposes. Genome editing by ...

Initially, T cell engineering depended on lentiviral or retroviral vectors to deliver DNA fragments into a cell for homologous recombination. Viral vectors allow for stable integration of DNA ...

The clones are available as glycerol stocks, plates, DNA minipreps or midipreps ... The constructs are in pLKO.1 lentiviral vector with puromycin selection. Carbenicillin selection is used for the ...